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Nuclei from Normal and Leukemic Mouse Spleen

I. The Isolation of Nuclei in Neutral Medium^*

(From the Sloan-Kettering Institute for Cancer Research, New York, N.Y.)

1. A procedure is reported for the isolation of mouse spleen nuclei in a neutral medium consisting of 0.88 M sucrose and 0.0018 M calcium chloride. The recovery of nuclei ranges from 10 to 20 per cent.

2. The isolated nuclei appear to be morphologically intact and free of cytoplasm and connective tissue.

3. These nuclei contain 6.5 mg. of DNA, 0.29 mg. of PNA, and 3.6 mg. of nitrogen per 10⁹ nuclei.

^* The authors wish to acknowledge the assistance of the National Cancer Institute of the Public Health Service, the Office of Naval Research, the Barker Welfare Foundation, and the James Foundation of New York, Inc.

The assistance of Miss Audrey M. Larack, who made the preliminary nucleic acid analyses, and of John Deonarine, who made the nitrogen analyses, is gratefully acknowledged.

Received 7/ 3/50.

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