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The Synthesis of Citrulline and p-Aminohippuric Acid by Rat Hepatoma^*

Ta Cheng Tung, M.D., Ph.D. and Philip P. Cohen, Ph.D., M.D.

(From the Laboratory of Physiological Chemistry, University of Wisconsin, Madison 6, Wis.)

1. Washed residue preparations of p-dimethylaminoazobenzene-induced hepatomas show a decrease in citrulline synthesis to approximately 14 and 30 per cent of normal in the presence of glutamic acid and carbamylglutamic acid, respectively. There is thus a relatively greater loss of enzyme activity in hepatoma tissue concerned with the conversion of glutamic acid to the carbamyl intermediate than for the conversion of the latter of citrulline. This loss of activity is due in part to the deficiency of some unknown essential in the diet of the experimental animals.

2. The synthesis of p-aminohippuric acid from p-aminobenzoic acid and glycine by washed residue prepared from hepatoma decreased to a level approximately 12 per cent of that of a similar preparation from normal liver.

3. The significance of these findings is discussed.

^* Aided in part by a grant from the National Cancer Institute of the National Institute of Health, Public Health Service.

Fellow of the Chinese Ministry of Education.

Received 8/10/50.