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Studies on the Proteins of Rhabdomyosarcoma and Normal Muscle of Mice. II. Electrophoretic and Viscometric Measurements

(From the Institute for Cancer Research and the Lankenau Hospital Research Institute, Philadelphia 11, Pennsylvania)

Exploratory electrophoretic and viscometric studies have been made of extracts of rhabdomyosarcoma and normal muscle of the mouse and of certain fractions obtained therefrom.

Electrophoretic measurements revealed seven major components in the tumor extracts and three major components in the muscle extracts. Mobility data indicated that none, except possibly one, of the major components of tumor is identical with any of those of normal muscle. Myosin preparations isolated from each type of tissue also differed in their mobilities. The possibility that some of the major components revealed by electrophoresis may represent groups of components was pointed out, and its significance was discussed.

The ribose-containing heavy material obtained from tumor tissue by centrifugation at 8,000 g was studied electrophoretically and found to be quite homogeneous.

Viscometric studies of the myosin of tumor and of muscle revealed that the former was significantly less viscous than the latter. The ribose-containing heavy material was found to be still less viscous. These results were interpreted to indicate that molecules of tumor myosin were less asymmetric than those of normal muscle myosin, while particles of heavy material were still less asymmetric.

Received 11/ 1/49.

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