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Some Effects of 2-Acetylaminofluorene on the Dog^*

(From the Bureau of Biological Research, Rutgers University, New Brunswick, New Jersey)

1. The carcinogen, 2-acetylaminofluorene, fed in a synthetic diet over a period of 6 months to dogs, caused a reduction in the protein stores of the body. The plasma albumin/globulin ratio, hemoglobin, red blood cells, liver nitrogen, and protein-free urinary nitrogen all decreased over the feeding period—variables which also decrease when animals are fed a protein-free diet.

2. The retention of dietary nitrogen, as measured by the nitrogen balance index also decreased below control values, demonstrating that the depletion in protein stores was the result of a reduction of retention of both dietary and body nitrogen.

3. A riboflavin depletion was produced in dogs fed 2-acetylaminofluorene. This depletion resulted in the appearance of typical skin lesions, in the reduction of liver riboflavin, and in an increase in the ability of the animal to retain excess injected vitamin. The carcinogen also reduced the excretion of riboflavin in the feces.

4. Hepatic neoplasms developed in three of the six dogs fed the carcinogen from 6 to 9 months. The urinary uric acid/allantoin ratio increased markedly in these dogs, an increase which was associated with the alteration in liver function.

^* These studies were supported by a grant from the Committee on Growth, American Cancer Society.

Received 12/10/49.

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