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Measurements of Ketoacids in Normal and Neoplastic Rat Tissue^*

(From the McArdle Memorial Laboratory, the Medical School, University of Wisconsin, Madison 6, Wisconsin)

1. A method is described for chromatography of ketoacids in the form of their 2,4-dinitrophenylhydrazones.

2. The method is used to determine pyruvic, oxalacetic, and -ketoglutaric acids in extracts of rat tissues which were frozen in situ with liquid air.

3. Analyses of tissues from animals frozen with and without nembutal anesthesia indicated the necessity of anesthesia to prevent changes due to stimulation of the tissues on contact with the liquid air.

4. Fasting was found to decrease these ketoacids in rat liver.

5. Pyruvic acid was found to be very low in normal tissues and somewhat elevated in venous blood and in neoplastic tissues.

6. Oxalacetic acid was very low in all tissues studied and was not detectable in blood or brain samples.

7. -Ketoglutaric acid was highest in kidney and in neoplastic tissues. The levels in most other tissues did not exceed that in blood.

^* This work was supported by a grant from the American Cancer Society on the recommendation of the Committee on Growth of the National Research Council.

Received 3/10/50.

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