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Macromolecular Particles Obtained from Human Neoplastic and Non-neoplastic Lymph Nodes

I. Procedure and Preliminary Results^*

Miriam S. Hoster, M.S., Bette J. McBee, B.A., Harry A. Rolnick, Ph.D., Quentin Van Winkle, Ph.D. and Herman A. Hoster, M.D.

(From the Departments of Medicine and Chemistry, The Ohio State University, Columbus 10, Ohio)

The segregation of dispersed cellular particles obtained from human lymph nodes has been carried out using a ten-step centrifugation procedure and a fluid medium consisting of 0.1 M phosphate buffer, pH 8.0, containing 0.03 per cent or 0.3 per cent heparin. Nuclear, mitochondrial, and submicroscopic particle fractions are obtained at suitable gravitational forces.

An analysis of the particle populations of the high gravity centrifugation sediments indicates the presence of polydispersed particles ranging from 10 to 280 mµ in neoplastic and non-neoplastic lymph nodes. The predominant particle size obtained from Hodgkin's lymph nodes, 10–20 mµ, differs significantly from the particle sizes found in the non-neoplastic lymph nodes.

The presence of ribose nucleic acid and the absence of desoxyribose nucleic acid was demonstrated in the high gravity sediments. The per cent nucleic acid in high gravity sediments of neoplastic and non-neoplastic lymph nodes were compared.

^* This investigation was supported by research grants from the National Cancer Institute of the National Institute of Health, Public Health Service, and the American Cancer Society.

Deceased September 9, 1949.

Received 1/ 2/50.