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Tracer Studies on the Metabolism of the Gardner Lymphosarcoma

IV. The Conversion of Lactate-2-C¹⁴ to Alanine, Glutamate, and Aspartate by Tumor and Spleen Cells^*

Saul Kit and David M. Greenberg

( Division of Biochemistry, School of Medicine, University of California, Berkeley, Calif.)

When mouse lymphosarcoma or spleen cells were incubated with lactate-2-C¹⁴, significant radioactivity was found in the respiratory CO₂. The only amino acids strongly labeled were alanine, glutamate, and aspartate; and the protein was also significantly labeled. Negligible radioactivity was found in the fats. In the spleen cells, the glutamate and aspartate contained almost equal radioactivity, while the alanine was less radioactive. On the other hand, in the lymphosarcoma cells the alanine had twice as much radioactivity and the aspartate one-fifth as much as the glutamate.

^* Aided by research grants from the American Cancer Society, on recommendation by the Committee on Growth of the National Research Council, the National Cancer Institute, Public Health Service, and the Hobson Fund of the Cancer Research Institute, University of California School of Medicine.

Predoctoral Research Fellow, National Cancer Institute, National Institutes of Health, Public Health Service, 1950–51.

Present address: Chemical Division, Dept. of Medicine, University of Chicago, Chicago, Ill.

Received 6/25/51.