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Studies on the Ehrilic Ascites Tumor

I. The Enzymic and Metabolic Activities of the Ascitic Cells and the Ascitic Plasma

E. Kun^*, P. Talalay and H. G. Williams-Ashman

( Ben May Laboratory for Cancer Research, Nathan Goldblatt Memorial Hospital for Neoplastic Diseases, Departments of Medicine and Surgery, University of Chicago, Chicago 37, Ill.)

Washed Ehrlich ascites tumor cells exhibited high rates of anaerobic and aerobic glycolysis which were dependent upon the addition of either glucose or fructose. Considerable oxygen uptake was observed when these cells were suspended in the absence of added substrate, and the endogenous respiration was suppressed on the addition of glycolyzable sugar, the respiratory quotient being elevated at the same time. The activity of a number of enzymes in homogenates of the tumor cells was studied, and mitochondria isolated from such preparations were shown to catalyze a marked uptake of inorganic phosphate concomitant with the oxidation of succinate. The rate of phosphorylation of various concentrations of glucose and fructose by the hexokinase present in aqueous extracts of acetone powders of the tumor was shown to parallel the rate of anaerobic glycolysis of these sugars by the intact tumor cells.

The ascitic plasma was found to contain a number of hydrolytic and glycolytic enzymes but did not possess the ability to glycolyze hexose diphosphate to lactate.

^* Present address: Departments of Medicine and Biochemistry, Tulane University, New Orleans, La.

Schwimmer fellow in cancer research.

Received 7/ 9/51.

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