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Electrophoretic and Ultracentrifugal Studies on the Soluble Proteins of Various Tumors and of Livers from Rats Fed 4-Dimethylaminoazobenzene^*

Sam Sorof and Philip P. Cohen

( Laboratory of Physiological Chemistry, University of Wisconsin, Madison, Wis.)

1. The soluble proteins of preneoplastic livers of rats fed 4-dimethylaminoazobenzene (DAB) and those of livers adjacent to DAB-induced tumors exhibit essentially the same electrophoretic properties as those of the livers of stock or basal diet-fed rats.

2. The soluble proteins of DAB-induced liver tumor and its mesenteric metastases show a marked reduction of the slowly migrating (veronal, 0.1 µ, pH 8.6) electrophoretic h components, and a great increase in the more rapidly moving A and N components.

3. The electrophoretic properties of the soluble proteins of DAB-induced liver tumor, its mesenteric metastasis, Jensen sarcoma, Walker 256 carcinosarcoma, and Flexner-Jobling carcinoma show essentially similar electrophoretic patterns. The slowly growing, chloroform-induced, transplantable mouse hepatoma 112 B yielded an electrophoretic pattern intermediate between those of non-neoplastic mouse liver and other tumors.

4. The soluble proteins of the livers of rats fed stock diet, basal diet, and liver tissue adjacent to DAB tumors exhibit similar velocity sedimentation properties. However, the soluble proteins of DAB-induced liver tumor contain two rapidly sedimenting components whose comparable regions in the non-neoplastic liver extracts are minor and ill-defined.

5. Some implications of these findings with regard to carcinogenesis are discussed.

^* Aided in part by research grants from the Wisconsin Alumni Research Foundation, and the National Cancer Institute, National Institutes of Health, Public Health Service.

Present address, National Cancer Institute, Bethesda, Md.

Received 1/ 8/51.

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