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Tracer Studies on the Metabolism of the Gardner Lymphosarcoma

I. The Uptake of Radioactive Glycine into Tumor Protein^*

Emmanuel Farber, Saul Kit and David M. Greenberg

( Division of Biochemistry, University of California School of Medicine, Berkeley, Calif.)

The incorporation of glycine-2-C¹⁴ into the protein of the Gardner lymphosarcoma cells has been studied. The process is not markedly affected by changes in the Ca⁺⁺, Mg⁺⁺, or K⁺ ion concentrations of the medium or by changes in the hydrogen ion concentration between pH 7 and 8. Below pH 6.5, the uptake is inhibited. Glycine uptake is stimulated by glucose and by bicarbonate and, in the presence of these, is linear for 2 hours. Metabolic inhibitors or conditions whereby the cells are disrupted almost completely abolished uptake.

^* Aided by research grants from the American Cancer Society, recommended by the Committee on Growth of the National Research Council, and the National Cancer Institute, Public Health Service.

Fellow of the American Cancer Society, 1947–49. Present address: Department of Pathology, Tulane University School of Medicine, New Orleans, La.

Abraham Rosenberg Research Fellow, 1949–50; Public Health Service Research Fellow of the National Cancer Institute, 1950–51. The work reported was taken from a thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biochemistry at the University of California.

Received 2/ 6/51.