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The Intracellular Distribution of Protein, Nucleic Acids, and Riboflavin in the Livers of Mice and Hamsters Fed 4-Dimethylaminoazobenzene^*

J. M. Price, J. A. Miller and E. C. Miller

( McArdle Memorial Laboratory, the Medical School, University of Wisconsin, Madison 6, Wis.)

1. Sucrose homogenates of the livers from albino mice or from hamsters fed either a semi-synthetic diet or the same diet plus the hepatic carcinogen 4-dimethylaminoazobenzene were separated by differential centrifugation into nuclear, large granule (mitochondria), small granule (microsome), and supernatant fluid fractions. Homogenates of the livers of C3H mice fed a stock diet were similarly fractionated. The unfractionated homogenates and the four fractions were analyzed for protein, nucleic acids, and riboflavin.

2. The livers from hamsters, which are refractory to the carcinogenic action of this aminoazo dye, were unaffected following ingestion of the compound for 4 months, except that there was an increase in the riboflavin content of the large granule fraction.

3. Mouse liver, which is slightly susceptible to the carcinogenic action of 4-dimethylaminoazobenzene, was altered considerably by the ingestion of this aminoazo dye for 4 months. There were decreased levels of protein, pentosenucleic acid, and riboflavin in the large granules comparable to those previously found in rat liver. However, the increase in the protein content and the decrease in the pentosenucleic acid content of the supernatant fluid were not found in liver from rats fed the dye.

4. The intracellular distribution of protein, nucleic acids, and riboflavin was essentially the same in normal mice, rats, and hamsters.

^* This work was supported in part by a grant from the National Cancer Institute, Public Health Service.

Predoctoral Research Fellow, Public Health Service, 1948–49.

Received 2/23/51.