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Studies on Lymphocystis Tumor Cells of Fish

II. Granular Structures of the Inclusion Substance as Stages of the Developmental Cycle of the Lymphocystis Virus

( Department of Anatomy, Woman's Medical College of Pennsylvania, Philadelphia 29, Pa.)

The formation of the osmiophilic granules, previously interpreted as elementary bodies of the lymphocystis virus (14), in the outer layer of the inclusion bodies of lymphocystis tumor cells is not an abrupt process. In stages preceding the appearance of the osmiophilic granules, a granular composition of the basophilic inclusion substance can be demonstrated in thin layers of typical inclusion bodies by the Sjövall osmium method as well as in swollen inclusions with ordinary methods of fixation and staining. In the osmium preparations the "granules of the inclusion substance" show a slighter staining reaction with osmic acid and a lower index of refraction than the osmiophilic granules. Evidence is presented suggesting that the granules of the inclusion substance multiply by fission and eventually form the osmiophilic granules. Thus, in the developmental cycle of the lymphocystis virus two types of particles of elementary size can be distinguished showing different features in osmium preparations: (a) the granules of the inclusion substance, interpreted as vegetative stages which serve for the growth of the virus within the host cells, and (b) the osmiophilic granules which are considered to be the virus particles which transmit the infection from host to host. Accumulated in large numbers in advanced lymphocystis cells, they are liberated when the tumor cells disintegrate in the water after extrusion or after the death of the fish.

Received 9/20/50.

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