This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Burchenal, J. H. Articles by Armstrong, R. A. Search for Related Content PubMed PubMed Citation Articles by Burchenal, J. H. Articles by Armstrong, R. A.

Sterilization of Leukemic Cells In vivo and In vitro^*

J. H. Burchenal, M. A. Cremer, B. S. Williams and R. A. Armstrong

( Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research, New York, N.Y.)

1. An in vivo technic for inactivating leukemic cells has been described as an aid in differentiating between modes of action of compounds chemotherapeutically active against transplanted mouse leukemia.

2. Six nitrogen mustards and two ethylenimines have been tested by this method against strains Ak4, Ak 9417, and Ak 1394 of mouse leukemia. With the exception of 2-ethylenimino-4,6-dimethoxy-s-triazine, all showed a definite sterilizing effect on leukemic cells.

3. 2,6-Diaminopurine and five antagonists of pteroylglutamic acid were tested and found to have no inactivating properties in vivo at dosage levels up to 50 and 100 times the LD₅₀.

4. Even when nephrectomized mice were used in an attempt to reduce the excretion of the drug, massive doses of 4-amino-N¹⁰-methyl-PGA did not sterilize the leukemic cells in vivo.

5. An in vitro technic was also described in which methylbis (2-chlorethyl)amine and 2,6-diaminopurine sterilized leukemic cells at concentrations corresponding to 1–4 times the LD₅₀ dose for the intact animal. By this method, however, 4-amino-N¹⁰-methyl-PGA had no effect even at concentrations corresponding to 50–100 times the LD₅₀ dose.

^* This investigation was supported, in part, by a research grant from the National Cancer Institute of the National Institute of Health, United States Public Health Service, and, in part, by a research grant from the American Cancer Society.

Damon Runyon Senior Clinical Research Fellow.

Received 4/26/51.

This article has been cited by other articles:

				C.-S. WRIGHT, B. SUNDHARAGIATI, J. A. BASS, and A. E. BUNNER REVIEW OF THE 1952 HEMATOLOGY LITERATURE Arch Intern Med, September 1, 1953; 92(3): 357 - 437. [Abstract] [PDF]