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Cultivation of 4-Dimethylaminoazobenzene-induced Rat Liver Tumors in Yolk Sacs of Chick Embryos^*

M. I. Armstrong, A. E. Gray and A. W. Ham

( Departments of Anatomy and Physics, University of Toronto, Toronto, Canada)

Of 35 4-dimethylaminoazaobenezene-induced rat liver tumors introduced into the yolk sacs of fertile eggs, seven infected the eggs. Of the remaining 28, four of the seven that had metastasized lent themselves to successful serial cultivation, while only one of the 21 that had not metastasized could be serially grown.

Three of these tumors have now been transferred in yolk sacs 94, 93, and 64 times, respectively, and have shown no change in their histological pattern. All three tumors, however, on serial transfer, have become increasingly lethal for chick embryos.

Rat liver tumors cultivated in yolk sacs have many advantages over primary tumors obtained from livers; they are of a consistent pattern, have only a little stroma, and contain no other parenchyma than tumor cells. Moreover, since only liver tumors that have metastasized generally grow in yolk sacs, their malignancy is probably assured.

^* This work was supported by grants from the National Cancer Institute of Canada and the Foster Bequest Fund of the University of Toronto.

Graduate Research Fellow, National Cancer Institute of Canada.

Received 4/ 3/52.