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Nuclei from Normal and Leukemic Mouse Spleen. III. The Desoxypentosenucleic Acid Content per Nucleus Calculated from Total Cell Counts^*

( Sloan-Kettering Institute for Cancer Research, New York, N.Y.)

1. A procedure for making total cell counts on mouse spleen has been described.

2. The desoxypentosenucleic acid (DNA) contents per nucleus calculated from the DNA contents and cell counts on the whole tissues were 6.6 x 10^-9 mg. for normal spleen; 7.3 for spontaneous leukemia; 7.4 for transplanted leukemia, line AK4; and 7.5 for line 9421.

3. The same values were obtained for the DNA content per nucleus either by analysis and cell counts of whole spleen or by analysis and counts of isolated nuclei. This suggests that, when nuclei are isolated in a neutral sucrose-calcium chloride medium, no DNA is lost during the isolation procedure and that the isolated nuclei are representative of the total population.

4. The number of cells per gram of spleen was 22.9 x 10⁸ for normal spleen; 17.5 x 10⁸ for spontaneous leukemia; 15.7 x 10⁸ for AK4 leukemia; and 14.1 x 10⁸ for 9421 leukemia.

^* The authors wish to acknowledge the assistance of the National Cancer Institute of the United States Public Health Service and the Atomic Energy Commission (Contract AT[30-1]-910).

Received 6/11/52.