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Electrophoretic Studies on the Water-soluble Proteins of Liver during Azo Dye Carcinogenesis in the Rat^*

( Biochemical Laboratory, Department of Chemistry, Stanford University, Stanford, Calif.)

1. The livers of normal rats and of those in advanced 3'-methyl-4-dimethylaminoazobenzene-induced preneoplasia, were extracted at pH 5; similar extracts were prepared from hepatomas, from blood serum from hepatoma-bearing rats, and from a methylcholanthrene-induced sarcoma.

2. The electrophoretic diagrams of the extracts from normal and preneoplastic liver were similar in phosphate buffer (pH 7.7; /2, 0.1).

3. The hepatoma extracts differed from the liver extracts in having less of the more slowly moving components, and more of the faster moving ones.

4. The electrophoretic diagrams of hepatoma extracts resembled those of blood serum in respect to the mobilities of the components but differed in respect to composition in both phosphate buffer (pH 7.7; /2, 0.1) and veronal buffer (pH 8.6; /2, 0.1). In the hepatoma extracts there was less of a component with the mobility of serum albumin, and more of one with the mobility of serum ₂-globulin.

5. The electrophoretic diagrams of extracts of hepatoma and sarcoma were similar in both buffers; however, the sarcoma extract showed an increase in the component with the mobility of serum ₁-globulin, while the ₂-globulin was increased in the hepatoma extract.

^* This investigation was supported by a research grant from the National Cancer Institute, of the National Institutes of Health, Public Health Service.

Received 6/ 8/52.