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The Effects of Freezing, Storage, and Thawing Upon the Transplantability of Mouse Leukemic Cells^{* ,}

Ronald M. Gabrielson, Jerome T. Syverton and Arthur Kirschbaum

( Department of Bacteriology and Immunology and the Department of Anatomy, University of Minnesota, Minneapolis, Minn.)

The success of transplantability of three lines of strain F leukemia cells was related to the duration of storage and to the rates of freezing and thawing. Tests for viability by transplantation of the treated leukemic cells to F₁ hybrid mice made it apparent (a) that freezing and thawing slowly had little or no effect upon the viability of the cells; (b) in contrast to the aforementioned findings, that freezing and thawing rapidly resulted in a total loss of viability when tested during the first 24 hours after trea ment, partial to total loss in the period from 36 to 60 hours, and positive transfer at 72 hours; (c) that the prolongation in the onset of leukemia in tests with rapidly frozen and thawed material resulted from the destruction of cells in numbers insufficient to interfere with the production of leukemia. The leukemic cellular suspensions, when tested before freezing, uniformly resulted, when transplanted to the control recipient mice, in leukemia.

^* This investigation was supported in part by a research grant from the National Cancer Institute of the National Institutes of Health, Public Health Service.

A preliminary report of this work was presented at the meeting of the American Association for Cancer Research at Cleveland, April 27–29, 1951 (Cancer Research, 11:249, 1951).

The material contained in this paper appeared in part in a thesis submitted in partial fulfillment of the requirements for the degree of Master of Science, and it will appear in part in a thesis to be submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the University of Minnesota.

Present address: Department of Anatomy, College of Medicine, University of Illinois, Chicago, Ill.

Received 10/ 4/51.