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In Vivo Studies on Incorporation of Glycine-2-C¹⁴ into Proteins and Nucleic Acid Purines^*

( McArdle Memorial Laboratory, The Medical School, University of Wisconsin, Madison 6, Wis.)

A kinetic study was made of the incorporation of glycine-2-C¹⁴ into proteins and nucleic acid purines of rat liver and tumor. From the subsequent breakdown of labeled moieties half-times were calculated. Determinations of specific activity of free glycine in the tissues were also carried out.

Isotopic glycine was incorporated into proteins less than hour after it was administered by stomach tube. In proteins of liver, Flexner-Jobling carcinoma, and kidney the peaks of specific activity occurred at about 12 hours.

The free purines were isolated from hydrolysates of PNA and DNA by paper chromatography. Radioactivity was first detectable in nucleic acid purines about 1 hour after the administration of glycine. In PNA and DNA purines from liver and tumor, peaks of specific activity occurred at about 24 hours; specific activity of DNA purines was somewhat lower than that of corresponding PNA purines. Nearly identical half-times were found for adenine and guanine in each nucleic acid fraction. Higher specific activities were reached in tumor for purines, in liver in the case of the proteins.

^* This work was supported in part by a grant from the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council and by a grant from the Wisconsin Section of the American Cancer Society.

Received 11/29/51.