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The Viscosimetric Determination of Desoxyribonuclease Inhibition^*

( Institute for Medical Research, Cedars of Lebanon Hospital, Los Angeles, Calif.)

1. A viscosimetric method is described for the determination of desoxyribonuclease activity and of the desoxyribonuclease inhibitor.

2. The inhibitory activity of cell extracts is optimum at pH 6.75. At acid pH values, interaction between the inhibitor and the enzyme is prevented, but the inhibitor is not destroyed. The reaction between the inhibitor and enzyme is instantaneous and reversible.

3. Extracts of human white blood cells and bone marrow cells, in the presence of plasma, show no significant losses of inhibitor activity over a 4-7-hour period, but gradually lose their activity over a period of 24 hours or more.

4. The inhibitor content of human serum is low, about 0.4 per cent/0.001 ml, and probably arises from the white blood cells.

^* Aided by a grant from the Charles R. Blakely Fund of the National Research Council.

Received 9/ 4/51.

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