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Effects of the Application of Carcinogens to Exposed Gastric Mucosa in the Rat^*

( Department of Pathology and the Experimental Surgical Laboratories, McGill University, Montreal, Canada)

1. Experiments in which flaps of gastric mucosa of 200 rats were transplanted to the muscle of the anterior abdominal wall were described. These animals were divided into: two groups of untreated controls; one group treated by the implantation of threads impregnated with 20-methylcholanthrene in the flaps; one group in which the flaps were painted with 20-methylcholanthrene; one in which the flaps were painted with 2-acetylaminofluorene; and one group painted with polyethylene glycol 300, the vehicle used to dissolve the carcinogens. The tissues were studied histologically as the animals died. Observations extended beyond 1 year in some animals.

2. The mode of development of the flaps was described in detail, with emphasis on the characteristic occurrence of marked, recurrent ulceration and regeneration of the mucosa and the polyploid mode of growth.

3. No true neoplasms were observed in any of the groups, although deep proliferations of epithelium occurred which could be mistaken for neoplasms unless studied in serial section.

4. Metaplastic bone formation in the flaps occurred, the result of chronic inflammation, but was not confined to the carcinogen-treated groups.

5. Implantation of threads into the flaps was not an effective means of introducing carcinogens, since the threads disappeared early in the experiments.

6. Simple painting of the flaps with carcinogens was deemed ineffective in view of the recurring ulceration of the mucosa. Experiments with different means of introducing carcinogens were considered necessary before the efficacy of the method in producing experimental adenocarcinoma of the stomach could be finally evaluated.

^* This work was supported by a grant-in-aid from the National Cancer Institute of Canada.

Received 4/23/52.