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Studies on Tissue Metabolism by Means of in Vivo Metabolic Blocking Technics

I. A Survey of Changes Induced by Malonate in Tissues of Tumor-bearing Rats

( McArdle Memorial Laboratory, Medical School, University of Wisconsin, Madison, Wis.)

1. The acid profiles for a number of tissues of tumor-bearing rats indicate the variability of the acidic components from tissue to tissue. Two peaks of acidity were found in control samples of the blood, three in the heart, lung, thymus, spleen, and tumors, four in the brain, seven in the liver and nine in the muscle.

2. In animals treated with malonate, succinate accumulated in tumors to an extent comparable to the other tissues, i.e., 3–5 µM per gram wet weight in 2 hours.

3. In most tissues of malonate-treated rats, the content of the peaks containing amino acids either remained constant or increased; in the Flexner-Jobling tumor, a marked decrease was noted in titration, amino acid content, and content of glutamate and aspartate, corresponding to the increase in succinate.

^* Public Health Service post-doctoral research fellow of the National Cancer Institute.

Received 5/ 1/52.