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The Effect of Nitrogen Mustard on the Cellular Concentrations of Nucleic Acids in Regenerating Rat Liver^*

John E. Ultmann, Erich Hirschberg and Alfred Gellhorn

( Institute of Cancer Research and Departments of Biochemistry and Medicine, Columbia University, College of Physicians and Surgeons, New York 32, N.Y.)

1. The suitability of regenerating rat liver for studies on the biochemical mechanism of action of cancer chemotherapeutic agents has been evaluated.

2. In agreement with data obtained by other investigators, it was found that there was an accumulation of nucleic acids in the liver cells in the early periods of regeneration following partial hepatectomy. This accumulation reached its peak 36 hours after hepatectomy.

3. The subcutaneous injection of a single dose of nitrogen mustard was followed by a significantly greater accumulation of nucleic acids in the liver cells at 36 hours after hepatectomy. At 48 and 60 hours, there was no longer a demonstrable difference in the nucleic acid levels per cell between treated and untreated animals.

4. It appears that in this biological system the inhibition of mitosis by nitrogen mustard was not mediated primarily through an inhibition of nucleic acid synthesis, as suggested by the work of other investigators with isotopically labeled precursors of nucleic acids.

^* This investigation was supported in part by a research grant (C-1386) from the National Cancer Institute, of the National Institutes of Health, Public Health Service.

Present address: New York Hospital, New York 21, N.Y.

Received 8/18/52.

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