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The Distribution of Stilbamidine in the Livers of Normal and Sarcoma-bearing Mice^*

( Radiation Laboratory and Donner Laboratory of Medical Physics, University of California, Berkeley, Calif.)

The radioactivity present in the livers of normal and sarcoma-bearing mice after injection of stilbamidine-amidine-C diisethionate has been shown to be present primarily in the mitochondria isolated by differential centrifugation in 0.25 M or 0.88 M sucrose. Subsequent disintegration of the mitochondria by sonic disintegration, or by release of gas pressure, released a large amount of the protein and dialyzable constituents into solution but only a small amount of the radioactivity. The particulate material remaining contained most of the stilbamidine and the ribonucleic acid of the mitochondria. However, experiments with ribonuclease and with trypsin and chymotrypsin on this fraction and on undisintegrated mitochondria indicate that the radioactivity was not associated with the ribonucleic acid or with the soluble, or perhaps even with the insoluble, protein of the mitochondria. Indeed, even though no clear evidence for the exact nature of the retention of the stilbamidine has been obtained, it appears most likely to be an association, possibly adsorption, with the membrane or particulate material of the mitochondria.

^* The work described in this paper was sponsored by the United States Atomic Energy Commission.

Received 9/ 9/52.