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Enhanced Tumor Transplantability Following Second Transplantation in Mouse of Original Strain

( Departments of Pathology of the Birmingham Baptist Hospitals and the Medical College of Alabama, Birmingham, Ala.)

E 0771/Jax mammary carcinoma was inoculated subcutaneously into the groins of 87 C57BL/6Jax mice (original strain), and each died from tumor growth (100 per cent). There was no difference in the mortality in days after transplant or in the "percental accelerant index" (22), the 50 per cent point in the mortality curve, among the 69 inoculated singly and the eighteen inoculated a second time 12–14 days after the first.

Among BALB/cJax mice given subcutaneous inoculations in the groin of E 0771/Jax mammary carcinoma, four of 121 died (3 per cent) when given tumor taken from C57BL/6Jax mice singly inoculated, whereas twelve of twenty died (60 per cent) when given tumor taken from C57BL/6Jax mice doubly inoculated 12–14 days apart (P = 0.001, statistically sig.). The E 0771 tumor from the doubly inoculated mice is being transplanted serially, and thus far in two to four serial passages seventeen of 54 BALB/c mice so inoculated have died from the tumor (P = 0.01, sig.).

Among 57 hybrid mice (C57BR/cd x BALB/c) given E 0771 taken from doubly inoculated C57BL/6Jax mice, fifteen have died from the tumor in primary and serial transplants (26 per cent), whereas only one among 22 hybrid controls (4 per cent) inoculated with tumor from singly inoculated C57BL/6Jax mice has died from tumor growth (P = 0.03, prob. sig.).

Thus, the resistance of a foreign strain of mice to a tumor from an inbred line has been broken by the single expedient of obtaining the tumor used for transplant from a doubly inoculated mouse.

^* This work was supported by the special research and development fund of the Baptist Hospital Laboratory. The assistance of Nell Branson, Edmund Dowling, Bruce A. Elrod, Ann Foster, Lance Franklin, L. H. Kwong, F. M. Schabel, Jr., and James Ivory is gratefully acknowledged.

Received 5/29/53.