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The Production of L(+) Lactic Acid by Ehrlich Mouse Ascites Tumor Cells^*

( Cancer Research Institute of the New England Deaconess Hospital, Boston, Mass.)

Ehrlich mouse ascites tumor cells were incubated with glucose under an atmosphere of nitrogen for 5 hours, after which time the lactic acid formed was extracted, crystallized, and characterized as the zinc salt. Data on water of crystallization, carbon, hydrogen, and ash analyses, and optical rotation indicate that the tumor produced L(+) lactate.

^* This work done under U.S. Atomic Energy Commission Contract AT(30-1)-901 with the New England Deaconess Hospital.

Received 7/ 8/53.