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Studies on the Metabolism of Acetate-1-C¹⁴ in Tissues of Tumor-bearing Rats^*

( Departments of Biochemistry and Pathology, Yale University School of Medicine, New Haven, Conn.)

1. In further development of chromatographic technics, an apparatus was constructed for simultaneous analysis of eight tissue samples. Chromatographic separation of a mixture of acetic, glutamic, aspartic, lactic, and formic acids was achieved in a system in which acetic acid was the eluting agent and Dowex-1 in the acetate form was the stationary phase.

2. With the above technics, further studies on the metabolism of acetate-1-C¹⁴ in tumor-bearing animals were carried out. In a series of tumors of malonate-treated animals, the specific activity of the succinate accumulated was 1/20th that of the liver, kidney, and spleen, 8 minutes after the injection of the tracer.

3. While most tissues of tumor-bearing animals converted 70–100 per cent of the total radioactivity reaching the tissue into nonvolatile compounds, the bulk of the radioactivity in the tumors persisted as acetate.

4. When acetate-1-C¹⁴ was injected into animals that were untreated with malonate, 30–60 per cent of the total radioactivity was found in glutamate in tissues other than tumors, while, in the tumors and blood, about 10 per cent of the total radioactivity was found in this substance 3 minutes after the injection of the tracer.

5. In the untreated animals, 8 minutes after intraperitoneal injection of acetate-1-C¹⁴ and 3 minutes after intravenous injection of the tracer, the percentage of isotope transferred to other compounds was approximately the same as that in the malonate series.

^* This investigation has been aided by grants from the Jane Coffin Childs Memorial Fund for Medical Research and from the Anna Fuller Fund.

Received 6/22/53.