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In Vitro Incorporation of Glycine-2-C¹⁴ into Purines and Proteins^*

( McArdle Memorial Laboratory, University of Wisconsin, Madison, Wis.)

In vitro experiments with whole-cell preparations of Ehrlich carcinoma, Gardner lymphosarcoma, and mouse liver led to extensive incorporation of glycine-2-C¹⁴ into the cell proteins and nucleic acid purines. With homogenates the extents of incorporation were much lower. The acid-soluble purines had a higher order of radioactivity than those of the nucleic acids. The addition of various purine compounds to the medium led to dilution of the radioactivity reaching the nucleic acid purines in certain cases. This dilution took place in both DNA and RNA, but to a larger extent in the RNA. The amounts of incorporation were equally high under aerobic or anaerobic conditions and were routinely studied under anaerobic conditions.

^* This research was supported in part by a grant from the American Cancer Society on the recommendation of the Committee on Growth of the National Research Council and in part by the Alexander and Margaret Stewart Fund.

Received 10/17/52.