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Enzymatic and Chemical Studies on the Livers and Liver Mitochondria of Rats Fed 2-Methyl- or 3'-Methyl-4-dimethylaminoazobenzene

( National Cancer Institute, National Institutes of Health, Bethesda, Md.)

1. The livers of rats fed either a commercial chow diet, a semi-synthetic basal diet, as such, or supplemented with the noncarcinogenic azo dye, 2-Me-DAB, or the carcinogen, 3'-Me-DAB, were studied with respect to their nucleic acid content and their succinoxidase, DPN-cytochrome c reductase, octanoxidase, uricase, DNase, and RNase activities. The mitochondrial fractions of these livers were also isolated and analyzed for nucleic acids and enzymatic activity.

2. With the exception of DNase activity, the activity of the enzymes studied was lower in the livers of the rats fed the basal diet than in the livers of the rats on the chow diet. There was also less mitochondrial material in the basal livers than in the livers of the rats fed the chow diet.

3. The succinoxidase and DPN-cytochrome c reductase activity of the 2-Me-DAB livers was greater than that of the livers of the rats fed the basal diet, while the activity of the other enzymes studied was about the same in the 2-Me-DAB and basal livers. The amount of mitochondrial material was increased in the 2-Me-DAB livers, while the DNA and PNA concentrations in these livers was decreased slightly.

4. The DNase and RNase activities and the DNA concentration were considerably elevated in the livers of the rats fed 3'-Me-DAB, while the activity of the other enzymes studied was decreased.

5. The differences in the nucleic acid concentrations and enzymatic activities in these livers were correlated with and discussed in relation to the data on the number of mitochondria and nuclei and the quantitative morphological data presented in the previous paper.

Received 11/10/52.