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Effect of Dose and Hormones on Tumor Production in Rats Given Emulsified 9,10-Dimethyl-1,2-benzanthracene Intravenously^*

( Department of Nutrition, Harvard School of Public Health, Boston 15, Mass.)

Tumor production in female rats by means of intravenously administered 9,10-dimethyl-1,2-benzanthracene (DMBA) has been further studied from the standpoint of the dose of carcinogen given and the influence of simultaneously administered -estradiol or diethylstilbestrol. Administration of approximately 5.2 mg of DMBA/100 gm body weight produced a tumor incidence of approximately 90 per cent in 19 weeks, in contrast to an incidence of 80 per cent in 47 weeks with a dose of 1.25 mg. The rats which received the higher dose of carcinogen had twice as many tumors per rat as the others.

The simultaneous administration of 0.6 mg. of estradiol caused an earlier appearance of tumors, increased the tumor incidence to 100 per cent in about 16 weeks, increased the number of tumors per rat, and yielded mammary adenocarcinomas almost exclusively. Six-tenths milligram of diethylstilbestrol was almost as effective as estradiol. Neither hormone alone in doses up to 4 mg/100 gm body weight produced any tumors.

^* Supported in part by grants-in-aid from the National Cancer Institute, National Institutes of Health, United States Public Health Service, Bethesda, Md.; from the American Cancer Society through an institutional grant to Harvard University; and from The Nutrition Foundation, Inc., N.Y.

Received 1/31/53.

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