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Studies in 2-Acetylaminofluorene Carcinogenesis

I. The Intracellular Distribution of Nucleic Acids and Protein in Rat Liver^*

( Department of Biochemistry and the Division of Endocrine and Cancer Research, Jefferson Medical College, Philadelphia, Pa.)

1. The course of AAF carcinogenesis (liver) involved a progressive increase in the percentage of cell protein and pentosenucleic acid in the nuclear fraction and a concomitant, but more drastic, reduction of the fraction of these constituents associated with the mitochondria.

2. During the preneoplastic period, no increases were noted in DNA, but an increase of 50 to 100 per cent, corresponding to the increased cellularity, was observed in the tumor.

3. Alterations in the chemical composition of the nucleus and mitochondria were observed during carcinogenesis. These resulted in an increase in the protein/DNA and the PNA/DNA ratios of the nucleus.

4. The alterations in cellular morphology and composition have been discussed from the point of view of a self-accelerating disturbance in the cytoplasmic-nuclear relations leading to the establishment of a new balance in the neoplastic state.

^* This work was supported in part by a research grant from the National Cancer Institute, National Institutes of Health, U.S. Public Health Service. We are indebted to Mrs. Edith Gollub for invaluable technical assistance.

Received 10/ 1/53.