This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rutman, R. J. Articles by Paschkis, K. E. Search for Related Content PubMed PubMed Citation Articles by Rutman, R. J. Articles by Paschkis, K. E.

Studies in 2-Acetylaminofluorene Carcinogenesis

II. The in Vitro Uptake of Alanine-1-C¹⁴ by Preneoplastic Liver and Hepatoma Mitochondrial Protein^*

( Department of Biochemistry and the Division of Endocrine and Cancer Research, Jefferson Medical College, Philadelphia, Pa.)

1. The stimulation of in vitro incorporation of C¹⁴-labeled amino acids into mitochondrial proteins by factors in the mitochondrial-free supernatant and by ATP has been confirmed.

2. Preneoplastic and hepatoma mitochondria showed increases of approximately 50 per cent and 200 per cent, respectively, in uptake activity when compared to normal liver preparations.

3. Limited but suggestive evidence has been noted of a wide divergence in the pattern of incorporation of C¹⁴ between preneoplastic and normal preparations.

4. The enhanced incorporation has been discussed from the point of view of compatibility with the protein synthetic requirements of AAF carcinogenesis.

^* This was supported in part by a research grant from the National Cancer Institute, National Institutes of Health, U.S. Public Health Service. We are indebted to Mrs. Edith Gollub for invaluable technical assistance.

Received 10/ 1/53.