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Certain Effects of 2-Aminofluorene and - and ß-Naphthylamines on Tetrahymena Pyriformis^*

Jay S. Roth

( William Goldman Isotope Laboratory, Department of Biological Chemistry, Hahnemann Medical College, Philadelphia, Pa.)

1. 2-Aminofluorene in concentrations as low as 5.5 x 10^-6 M inhibited the growth of Tetrahymena pyriformis W in proteose-peptone medium. In a synthetic medium containing Tween 80 growth was increased somewhat with concentrations ranging from 2.6 x 10^-5 to 3.95 x 10^-4 M. Higher concentrations were inhibitory.

2. 2-Aminofluorene decreased the respiration of Tetrahymena by 44 per cent at the end of an hour. Cystine, homocysteine, riboflavin, and riboflavin phosphate had no effect on the inhibitory action, but pantothenic acid reversed it by 50 per cent, while a liver extract completely reversed it.

3. The succinate-oxidizing activity of Tetrahymena homogenates was reduced 40 per cent by saturated solutions of 2-aminofluorene, 90 per cent by -naphthylamine, and 30 per cent by ß-naphthylamine. Ribonuclease and desoxyribonuclease activity were unaffected by 2-aminofluorene.

^* This project was supported by a grant from the American Academy of Arts and Sciences.

Present address: Strangeways Research Laboratory, Cambridge, England.

Received 12/ 1/53.