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The Transplantation of Small Numbers of Tumor Cells

( Chemical and Biological Research Section, Lederle Laboratories Division, American Cyanamid Company, Pearl River, N.Y.)

A method was developed for isolating small populations of tumor cells by means of the double slide culture tube technic.

Known numbers of cells derived from mouse glioblastoma 8110 and the Walker rat carcinoma were transplanted, attached to the plasma film on which they were growing, under the skin of the appropriate strain of rat or mouse.

Glioblastoma 8110 was successfully transplanted with as few as two cells. Fifty to 60 per cent takes were obtained with cell numbers ranging from two to 80.

The Walker rat carcinoma was transplanted successfully in only five out of 165 attempts. The successful transplants had cell counts ranging from five to 72.

^* Present address: Department of Microbiology, University of Miami, South Miami 43, Florida.

Received 5/14/54.