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Chemical and Enzymatic Changes Associated with Mouse Liver Necrosis in Vitro^*

M. Berenbom, P. I. Chang, H. E. Betz and R. E. Stowell

( Department of Pathology and Oncology, University of Kansas Medical Center, Kansas City 12, Kansas)

Chemical and enzymatic changes have been studied in mouse liver undergoing necrosis or autolysis in vitro by incubating pieces of liver under controlled conditions of temperature, humidity, and sterility. Succinoxidase and cytochrome oxidase activity disappeared in 24 hours, while loss of activity of acid and alkaline phosphatase, esterase, and peptidase continued over a period of 72 hours. Free amino acids, acid-soluble nitrogen, and acid-soluble phosphorus increased up to 48 hours and then declined. Protein nitrogen, PNA, and DNA decreased steadily up to 48 hours and then remained relatively constant. PNA and DNA fell to much lower levels than the other constituents studied; the lipid content changed the least.

^* This investigation was supported by grants from the Atomic Energy Commission and the National Cancer Institute, U.S. Public Health Service.

Visiting Assistant Professor of Pathology, University of Kansas, 1953, from the Department of Pathology, University of Liége, Belgium.

Received 3/29/54.