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Growth of Mengo Encephalitis Virus in Ehrlich Ascites Cells in Vitro

( Viral and Rickettsial Section, Research Division, American Cyanamid Company, Pearl River, New York)

A simple tissue culture method has been described by which Mengo encephalitis virus can be propagated in Ehrlich ascites cells. The carcinoma cells can be maintained for a period of at least 48 hours in a completely defined synthetic medium. The greatest yield of virus was obtained when a ratio of 1 million Ehrlich ascites cells to 1 mouse LD₅₀ of Mengo encephalitis virus was employed. Preliminary results of some metabolic studies of normal and Mengo-infected ascites cell cultures are reported.

Received 4/ 1/55.