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Patterns of Free Amino Acids in Growing and Regressing Tumors^*

( Dept. of Biochemistry, Division of Research, City of Hope Medical Center, Duarte, California, and Roscoe B. Jackson Memorial Laboratory, Bar Harbor, Maine)

1. Determinations were made by two-dimensional paper chromatography of free amino acid patterns in tumor C1498 in both solid and ascites forms at various times after transplantation into C57BL/10-H-2^b mice, a strain in which the tumor grows progressively and kills the animals, and into C57BL/10-H-2^d mice, a subline which differs from the former by a single histocompatibility gene and in which the tumor grows initially and then regresses.

2. The amino acid patterns of the solid form of the tumor were virtually identical in both sublines for the first 8 days after transplantation. Subsequently, the patterns diverged, the tumors in the resistant strain showing a relative increase in free glutamic acid content together with the appearance of free glutamine, an amino acid not detected at any time on chromatograms of extracts from tumors grown in the susceptible subline.

3. Observations similar to those above were made with an ascites form of tumor C1498 grown in the two sublines of mice. Glutamine appeared in the cells and ascites fluid in the resistant strain but was not detectable in the fluid of the susceptible mice. Increases were noted in contents of several amino acids at later time-intervals of growth in the tumor cells in the resistant mice.

^* This investigation was supported by research grants C-1245 and C-2568 from the National Cancer Institute, United States Public Health Service.

Received 7/16/55.