This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Creech, H. J. Articles by Andre, J. Search for Related Content PubMed Articles by Creech, H. J. Articles by Andre, J.

Immunological Properties of Carcinogen-Protein Conjugates Containing Polycyclic Aromatic Hydrocarbons and Substituted Stilbenes^*

( Institute for Cancer Research and Lankenau Hospital Research Institute, Philadelphia 11, Pa.)

Antibodies toward 9,10-dimethyl-1,2-benzanthryl-3-carbamido horse serum albumin reacted well with heterologous proteins containing haptens which were either similar in size and configuration (3,4-benzpyrene) or smaller (1,2-benzanthracene, 2-acetylaminofluorene), but poorly with those containing a larger structure (1,2,5,6-dibenzanthracene). The appreciable structural alteration produced by photo-oxidation of 9,10-dimethyl-1,2-benzanthracene was reflected by a definite change in haptenic reactivity. Conjugates in which 2'-methyl-4-dimethylaminostilbene was joined to protein either by an azo or carbamido linkage showed haptenic activity and cross reactivity, but the two linkages were not equivalent. The terminal dimethylaminophenyl portion of the substituted stilbene molecule was found to be of considerable serological importance. After exhaustive absorption of the antihapten-protein serum by native protein and by hapten-heterologous portein, there still remained much antibody which was specifically precipitated by the completely homologous test antigen.

^* This research was supported in part by a Grant-in-Aid from the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council.

Received 7/ 8/55.