This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rumsfeld, H. W. Articles by Baumann, C. A. Search for Related Content PubMed PubMed Citation Articles by Rumsfeld, H. W., Jr. Articles by Baumann, C. A.

Proteinuria in Tumor-bearing Rats^*

( Department of Biochemistry, College of Agriculture, University of Wisconsin, Madison, Wis.)

1. Rats bearing hepatomas induced by 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) excreted more protein in the urine than normal rats. This increase was somewhat greater when the tumors were large. No increase in protein excretion was seen in rats bearing Flexner-Jobling adenocarcinomas or methylcholanthrene-induced sarcomas.

2. In confirmation of the work of others, electrophoretic analysis indicated that the urinary protein of normal male rats was predominantly globulin. The three major components had the mobilities of certain serum protein fractions: ₂-globulin, 50 per cent; albumin, 30 per cent; and ß-globulin, 17 per cent.

3. The presence of hepatomas in male rats was characterized by a shift of the urinary protein toward a higher percentage of the faster moving components. This shift was apparent at four pH's: 6.8, 8.0, 8.6, and 9.0. The shift became more pronounced as the hepatoma increased in size and eventually reached the following percentages in the urine of rats bearing large hepatomas: albumin, 47 per cent; ₂-globulin, 34 per cent; and ß-globulin, 15 per cent.

4. The alteration in the urinary protein of the hepatoma-bearing rat was not accompanied by any significant change in the serum protein pattern.

5. The urinary proteins from rats bearing the Flexner-Jobling adenocarcinoma or a methylcholanthrene-induced sarcoma were similar to urinary proteins from normal rats. Thus, the deviation from normal in the urinary protein from rats with hepatomas appeared to depend upon the hepatoma per se or upon the damaged liver rather than upon tumors in general.

^* Published with the approval of the Director of the Wisconsin Agricultural Experiment Station. Supported in part by a grant from the Committee on Growth, American Cancer Society, and from the Jonathan Bowman fund.

Received 8/15/55.