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The Incorporation of Glycine-2-C¹⁴ into Acid-soluble Nucleotide Purines^{* ,}

( McArdle Memorial Laboratory, University of Wisconsin, Madison 6, Wis.)

A short-term time study has been made of the in vivo incorporation of glycine-2-C¹⁴ into the acidsoluble purine nucleotides and into the nucleic acids of rat liver and Flexner-Jobling carcinoma. The acid-soluble nucleotides were more highly labeled than the corresponding nucleotides of the nucleic acids in both tissues. The tumor incorporated much more C¹⁴ into nucleotides than did the liver at all times studied.

Groups of acid-soluble purine nucleotides have been isolated from rat liver and Flexner-Jobling carcinoma at very short time intervals after the injection of glycine-2-C¹⁴. IMP consistently showed a higher specific activity than AMP, ADP, or ATP in both liver and tumor. GMP, GDP, and GTP were more highly labeled than the corresponding adenine nucleotides in tumor. GMP showed a higher specific activity than IMP in tumor. The total radioactivity in the individual nucleotides tended to be of the same magnitude. Using these data, various interrelationships among acid-soluble purine nucleotides have been discussed.

Acid-soluble nucleotides have been extracted from the nuclear and cytoplasmic fractions of Flexner-Jobling carcinoma. Gradient elution chromatography of the acid-soluble extracts of the two fractions indicate that similar nucleotides are contained in each fraction. Incorporation of glycine-2-C¹⁴ into corresponding nucleotides of each fraction showed some small but reproducible differences at early time intervals only. At later times the specific activities of the corresponding nucleotides in each fraction tended to be similar.

^* This research was supported in part by a grant from the American Cancer Society on the recommendation of the Committee on Growth of the National Research Council, in part by a grant from the Wisconsin Section of the American Cancer Society, and by the Alexander and Margaret Stewart Fund.

Preliminary Reports of this work have been published in Fed. Proc., 12:199, 1953; Fed. Proc., 13:202, 1954.

Received 8/16/54.