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Phosphorus Incorporation into Nucleic Acids and Proteins of Liver Nuclei of Normal and Azo Dye-fed Rats^*

Darrell N. Ward and A. Clark Griffin

( Department of Chemistry, School of Medicine, Stanford University, Stanford, Calif.)

The uptake of radioactive phosphate into the nuclear ribonucleic acid fraction from the liver of normal and precancerous animals has been studied. Measurements were made at -hour intervals following injection of the isotope for periods up to 4 hours, with further measurements at 5, 8, and 18 hours after administration. Liver nuclei were isolated, and the residue after acid and lipid extraction was used to isolate the nuclear ribonucleic acid as the constituent nucleotides following alkaline hydrolysis and paper chromatography.

The uptake of phosphorus into nuclear ribonucleic acid did not differ significantly in either the precancerous or control animals when compared with each other.

The constituent nucleotides did not differ significantly from one another in their uptake, except for adenylic acid, which was observed to be as much as 30 per cent higher than the other nucleotides in some instances.

The inorganic phosphate from phosphoprotein (presumably) liberated by hydrolysis with 14 N NH₄OH had a specific activity one-half to one-fourth as great as that liberated by a subsequent hydrolysis with 1 N NaOH.

^* Portions of this work were included in a thesis submitted to the Committee on Graduate Study, Stanford University, in partial fulfillment of the requirements for the degree of Doctor of Philosophy (20).

Atomic Energy Commission Predoctoral Fellow during the period in which this research was carried out. Present address, Department of Biochemistry, Cornell University Medical College, New York City.

Present address, Department of Biochemistry, University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas.

Received 2/14/55.