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Inhibition by Alcohols of Diseased Plant Growths in Tissue Culture^*

( Department of Plant Pathology, University of Wisconsin, Madison, Wis.)

Marigold, Paris-daisy, periwinkle, and sunflower tissue of crown gall and tobacco tissue of normal origin were incubated on synthetic media, with or without 2 per cent sucrose, but with 4, 2, 1, 0.5, 0.25, 0.125, 0.06, 0.03, 0.015, or 0 per cent butanol, dulcitol, ethanol, glycerol, mannitol, methanol, or propanol, with the exception of erythritol, for which the highest concentration used was 2 per cent. Without sucrose, marigold, Paris-daisy, and tobacco cultures grew only with glycerol. Sunflower, in addition, grew slightly with dulcitol, mannitol, or methanol. Periwinkle grew more or less with all the alcohols tested except butanol or propanol. With 2 per cent sucrose present in the culture medium, the five species showed variations with most of the alcohols. The amount of growth depended upon the species, the kind of alcohol, and its concentration. Propanol, however, inhibited the growth of most species even at the lowest concentration tested. Butanol was the next most inhibiting alcohol, followed by ethanol.

^* This work was supported in part by the Rockefeller Foundation, by the American Cancer Society, and by the Research Committee of the Graduate School from funds supplied by the Wisconsin Alumni Research Foundation. Published with the approval of the director of the Wisconsin Agricultural Experiment Station.

Received 3/14/55.