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Early Development of Squamous-Cell Carcinoma in Methylcholanthrene-painted Chicken Skin^*

( Department of Anatomy, Medical College of South Carolina, Charleston, South Carolina)

Fourteen light-brown Leghorn females were painted with methylcholanthrene (MC) on the ventral surface of the wing 4 times a week for 6 or 24 weeks. Twenty others received, in addition to MC, subcutaneous injections of a comb-mucoid extract (Mu). After 6 weeks of treatment two of the five MC-controls and three of the twelve MC + Mu-treated birds had carcinoma of the wing skin. Of the birds treated for 24 weeks, three of the nine MC-controls and two of the eight MC + Mu-treated wings developed carcinoma. The dermis of all treated wings was infiltrated with numerous large aggregates of lymphocytes.

The combs of eighteen capons were painted with MC 6 times a week for 34 weeks. Nine of these were stimulated by androgen. Carcinoma was observed in two of the combs not stimulated by androgen and in three of the androgen-stimulated combs. Aggregates of lymphocytes were predominant in unstimulated combs, but almost absent in androgen-stimulated combs.

In these experiments, the presence of combmucoid appeared to have no augmenting or inhibitory effect on carcinogenesis. Likewise, the presence of fowl pox lesions did not appear to be a contributory factor.

Although androgen-stimulated combs failed to show the typical lymphocytic reaction to MC, the injection of a comb-mucoid extract did not modify this response in MC-painted wings.

^* This investigation was supported by research grants C-2470 and RG-2829 from the National Institutes of Health, Public Health Service.

Received 3/23/55.