This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Pearson, B. Articles by Defendi, V. Search for Related Content PubMed PubMed Citation Articles by Pearson, B. Articles by Defendi, V.

Histochemical Studies of Succinic Dehydrogenase by Means of Tetrazolium in Rat Liver Tumors Induced by p-Dimethylaminoazobenzene^*

( Detroit Institute of Cancer Research and Department of Pathology, Wayne University College of Medicine, Detroit 1, Mich.)

The histochemical analysis of succinic dehydrogenase by means of a tetrazolium salt in azo dye-induced rat liver hepatoma has revealed the existence of two types of malignant cells, which have different histochemical patterns. The lower level of succinic dehydrogenase previously found by many authors in similar hepatoma by homogenate technic can be explained as an index of an average of general succinic dehydrogenase activity in a tissue, where part of the cells contain a fairly normal amount of this enzyme and part none at all.

In this series of azo dye-induced tumors we were unable with this histochemical technic to distinguish between benign and malignant cells.

^* This investigation was supported in part by research grant number C-2624 from the National Cancer Institute, U.S. Department of Health, Education, and Welfare, and in part by an Institutional Grant from the American Cancer Society.

Received 4/30/55.