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Distribution of Radiocarbon in Liver Choline, Creatine, and Methionine after the Injection of Methyl-C¹⁴-bis(ß-chloroethyl)amine^*

Eberhard G. Trams and Calvin T. Klopp

( George Washington University Cancer Clinic, Washington, D.C.)

Male adult rats were injected intravenously with methyl-C¹⁴-bis(ß-chloroethyl)amine HCl (HN2) over periods of 1–4 days. The amount of C¹⁴ contained in choline, creatine, and the S-methyl group of methionine isolated from the livers of these animals was determined. While the S-methyl group of methionine and creatine showed little, if any, incorporation of C¹⁴, 0.12–0.41 per cent of the total injected radioactivity was recovered in choline. Although incorporation of the labeled N-methyl group of HN2 into choline was demonstrated, it was concluded that transmethylation reactions do not represent major pathways in the intermediary metabolism of HN2.

^* This investigation was supported in part by a grant-inaid from the National Cancer Institute and by an Institutional Grant from the American Cancer Society.

Present address: Department of Pharmacology, George Washington University, School of Medicine, Washington, D.C.

Received 5/18/55.