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Growth and Continued Function of Rat Marrow Cells in X-radiated Mice^*

Peter C. Nowell, Leonard J. Cole, John G. Habermeyer and Patricia L. Roan

( Division of Biological and Medical Sciences, U.S. Naval Radiological Defense Laboratory, San Francisco 24, Calif.)

Leukocytes in rat blood and bone marrow smears stained black or brown when treated histochemically for alkaline phosphatase.

Mouse leukocytes in similar smears were uniformly phosphatase-negative.

Following intravenous injection of rat marrow cells into x-radiated (810 r) mice, phosphatase-positive cells were present in marrow smears within 2 hours.

With beginning marrow "regeneration" at 7 days, the phosphatase-positive cells rapidly increased in numbers.

By 14–28 days, when "regeneration" was nearly complete, essentially all the cells were phosphatase-positive, and the smears were indistinguishable from rat marrow.

Smears from mice injected with mouse bone marrow remained uniformly phosphatase-negative throughout all stages of regeneration.

These findings indicate that injected rat marrow cells survive, divide, and eventually repopulate the marrow cavity of irradiated mice. Prolonged survival of these mice, in some cases beyond 30 days, appears to be correlated with the continued growth of phosphatase-positive marrow. This suggests that transplanted rat marrow is actually functioning hematopoietically in the heterologous host.

^* Supported in part by funds from the Bureau of Medicine and Surgery, U.S. Navy Department. The opinions and assertions contained herein are the private ones of the authors and are not to be construed as official, or reflecting the views of the Navy Department.

Lt. (M.C.) U.S.N.R.

Received 11/ 4/55.

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