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The Chemistry of Experimental Chloroma

II. Isolation of Crystalline Protoporphyrin, Its Origin and Relation to Other Porphyrins^*

( Samuel S. Fels Research Institute, Temple University School of Medicine, Philadelphia, Pa., and University Minnesota Medical School, Minneapolis, Minn.)

Ethyl acetate-acetic acid extracts of chloroma tissue were found to contain thirteen chromatographic entities on calcium carbonate columns. Crystalline protoporphyrin was obtained from one of these. The ratio of porphyrin in the "copro" fraction to the porphyrin of the "proto" fraction was found to be constant in four different tissues. Administration of radioglycine-2-C¹⁴ resulted in greater uptake of C¹⁴ in porphyrin of chloroma than in hemes of the nontumor tissue, indicating the de novo synthesis of porphyrin in chloroma.

^* This investigation was supported by a research grant C-1966(C2) from the National Cancer Institute of the National Institutes of Health, Public Health Service.

Received 12/19/55.