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Ascites Tumor Development

II. Cytotoxicity of Various Antisera Prepared against Ehrlich ascites Tumor Cell Components^*

( Department of Zoology, Duke University, Durham, N.C., and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tenn.)

Antisera to whole Ehrlich ascites tumor cells and to components of these cells were prepared in rabbits and were tested, by their complement fixation activity, against the homologous and heterologous antigens. Ascites cell agglutination by these antisera was also compared. Both tests indicate wide cross-reactions among the antisera to the tumor cell components. The intraperitoneal injection of tumor-challenged mice with these antisera did not change their mean survival time. The survival of mice challenged with tumor cells that had been incubated with the different sera indicated a cytotoxic effect on the cells by the antisera to tumor cell mitochondria and to tumor cell nuclei. Phasecontrast microscopic observation of the living, treated cells revealed no significant change which might explain fully their altered growth when placed in a host.

^* This work was performed under Contract No. W-7405-eng-26 for the Atomic Energy Commission and was supported in part by research grants RG-4203 and RG-4203(c) from the National Institutes of Health, Public Health Service.

Received 2/11/56.