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Uptake of Radioactive Phosphorus in Experimental Tumors

I. Comparison of Radioactivity in Neoplastic and Normal Ocular Tissue^*

( Department of Surgery, Division of Ophthalmology, and Department of Radiology, Western Reserve University School of Medicine, and University Hospitals, Cleveland, Ohio)

Experimental intraocular tumors were produced in rabbits, guinea pigs, rats, and mice by transplantation of neoplastic tissue into the anterior chambers according to the technic developed by Greene. Animal tumors included the Murphy rat lymphosarcoma, the Brown-Pearce rabbit carcinoma, a rat glioblastoma (originally induced by implantation of 3-methylcholanthrene into the rat cerebrum), and the S-91 mouse melanoma. Heterotransplantation of tissue from fifteen different human cancers was also carried out in guinea pigs. Uptake of P³²—as measured with special Geiger counter probes—by the experimental intraocular tumors was compared in vivo and in vitro with that of normal ocular tissues in the same animal. In general, curves of counts per minute plotted against time of observation showed an initial rapid rise reaching a maximum within 10–12 minutes after injection of NaH₂PO₄, and then a decline, perhaps rapid at first, followed by a slow decline extending over several days. During the initial rise in radioactivity immediately after injection, blood levels of P³² also were high, and initial high counts were probably dependent largely upon tissue vascularity. After establishment of equilibrium in phosphorus exchange, curves of uptake declined and followed a gradual pattern of decay. These experimental results are parallel to those previously established by differential counts over ocular tumors in clinical studies.

^* This research was supported in part by a Federal Security Agency Public Health Service grant and performed in part under AEC contract #W-31-109-eng-78.

Received 3/29/56.