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Purification and Properties of a Nerve Growth-promoting Factor Isolated from Mouse Sarcoma 180^*

( Department of Zoology, Washington University, St. Louis, Mo.)

A heat-labile, nondialyzable factor with nerve growth-promoting properties has been partially purified from Sarcoma 180. The data indicate that the active material is a protein or is bound to a protein and that nucleic acids are not involved in its effectiveness. The factor must be continuously present in the cultures for the outgrowth of the nerve fibers to proceed. The outgrowth of nerve fibers was inhibited by iodoacetate, but not by cyanide, dinitrophenol, or fluoride.

^* This work has been supported by grant B-463 from the National Institute of Neurological Diseases and Blindness of the National Institutes of Health, Public Health Service; by a grant of the American Cancer Society, recommended by the Committee on Growth, National Research Council, and by a contribution from an Institutional grant of the American Cancer Society to Washington University. The able assistance of Miss Barbara Bankhead is gratefully acknowledged.

Received 7/23/56.

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