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Maintenance at Low Temperatures of the Ehrlich Ascites Carcinoma of the Mouse^{* ,}

( Langbord Virus Laboratory, Department of Microbiology, Hahnemann Medical College, Philadelphia, Pa.)

In studying the means of preserving the Ehrlich ascites tumor it was observed that the tumor could be maintained for at least 1 year by the addition of an equal volume of 40 per cent glycerol, and, after 1 hour, placing the stoppered preparation directly into a dry-ice chest. Another significant observation in this study was the lack of correlation between viability staining data and the recoverability of the ascites tumor.

^* Aided by a grant from the American Cancer Society.

Studies carried out during the tenure of a Lederle Medical Faculty Award.

Received 8/15/56.